Nasal Bradykinin Assay (PCS1)
See Collection and Handling of Nasal Lavage Samples for details on sample collection and preparation.
Bradykinins in nasal secretions were measured using high performance liquid chromatography (HPLC) analysis following the protocol described by Proud and colleagues1.
The nasal bradykinin assay for PCS1 was performed at the ENT Research Laboratory of the Children’s Hospital of Pittsburgh.
1 Proud, D., Togias, A., Naclerio, R. M., Crush, S. A., Norman, P. S., & Lichtenstein, L. M. (1983). Kinins are generated in vivo following nasal airway challenge of allergic individuals with allergen. Journal of Clinical Investigation, 72, 1678-1685.