Carnegie Mellon University

Hironobu Murata

Hironobu Murata

Senior Research Scientist

Address
Carnegie Mellon University
4400 Fifth Avenue
Mellon Institute
Pittsburgh, PA 15213

Education

04/1994 – 03/1997             Ph.D., Tokyo Institute of Technology, Japan

                                              Supervisor: Professor Takeshi Endo 

04/1992 – 03/1994             M.Sc., Tokyo University of Agriculture and Technology, Japan

                                              Supervisor: Professor Mitsuaki Narita

04/1990 – 03/1992             B.Sc., Tokyo University of Agriculture and Technology, Japan

                                              Supervisor: Professor Mitsuaki Narita 

Research

07/2018 – present               Senior Research Scientist

                                               Engineering Research Accelerator

                                               Carnegie Mellon University                                            

 

09/2012 – 07/2018              Research Scientist

                                               Engineering Research Accelerator

                                               Carnegie Mellon University

                                                                  

08/2004 – 08/2012              Research Associate

                                               McGowan Institute for Regenerative Medicine

                                               University of Pittsburgh

 

01/2002 – 12/2003              Postdoctoral Associate

                                               Department of Chemical Engineering

                                               University of California, Berkeley

 

03/2000 – 12/2001              Postdoctoral Associate

                                               Institute for Microsystem Technology (IMTEK)

                                               The Albert Ludwig University of Freiburg, Germany

 

04/1997 – 02/2000              Postdoctoral Associate

                                               Max-Planck-Institute for Polymer Research, Germany

 

Publications

46. “Rational Control of Protein-Protein Interactions with Protein-ATRP Generated Protease-Sensitive Polymer Cages", Kaupbayeva, B., Murata, H., Rule, G., Matyjaszewski, K., Russell, A.J., Biomacromolecules, Submitted. 

45. “A Comprehensive Analysis in One Run-in-Depth Conformation Studies of Protein-Polymer Chimeras by Symmetrical Flow Field-Flow Fractionation”, Kaupbayeva, B., Murata, H., Matyjaszewski, K., Boye, S, Russell, A.J., Lederer A., Chemical Science, 12, 13848 – 13856. (2021).

44. “Molecular Dynamics-Guided Design of a Functional Protein-ATRP Conjugate That Eliminates Protein-Protein Interactions”, Kaupbayeva, B., Boye, S., Munasinghe, A., Murata, H., Matyjaszewski, K., Lederer A., Colina,Colina, C.M., Russell, A.J. Bioconjugate Chemistry, 32, 821 – 823 (2021).

43. “Mesenchymal Stem Cells Coated with Synthetic Bone-Targeting Polymers Enhance Osteoporotic Bone Fracture Regeneration”, Safarova, Y., Olzhayev, F., Umbayev, B., Tsoy, A., Hortelano, G., Tokay, T., Murata, H., Russell, A.J., Askarova, S., Bioengineering, 7, 125 (2020).  

42. “Catalytic Detoxification of Organophosphorus Nerve Agents by Butyrylcholinesterase-Polymer-Oxime Bioscavengers”, Zhang, L., Murata, H., Amitai, G., Smith, P.N., Amitai, G., Matyjaszewski, K., Russell, A.J., Biomacromolecules, 21, 3867-3877 (2020).  

41. “Tuning Butyrykcholinesterase Inactivation and Reactivation by Polymer-Based Protein Engineering”, Zhang, LB.., Baker, S.L., Murata, H., Harris, N., Ji, W., Amitai, G., Matyjaszewski, K., Russell, A.J., Advanced Science, 7, article number 1901904 (2020). 

40. “Utilization of the Polymer Sieving Effect for the Remove of the Small Molecules Biotin-CDM”, Lucas, A., Kaupbayeva, B., Murata, H., Cummings, C., Russell, A.J., Minden, J.S., ACS Appl. Polym. Mater., 1, 2897-2906 (2019).  

39. “Transforming Protein-Polymer Conjugate purification by tuning protein”, Baker, S.L., Munasinghe, A., Kaupbayeva, B., Kang, N.R., Certiat, M., Murata, H., Matyjaszewski, K., Lin, P., Colina, C.M., Russell, A.J., Nature Communications, 10, article number 4718 (2019).

38. “Ligands and Characterization for Effective Bio-Atom Transfer Radical Polymerization”, Murata, H., Baker, S.L., Kaupbayeva, B., Lewis, D.J., Zhang, L.B., Boye, S., Lederer, A., Russell, A.J., Journal of Polymer Science Part A-Polymer Chemistry, 58, 42-47 (2019).  

37. “Charge-Preserving Atom Transfer Radical Polymerization Initiator Rescues the Lost Function of Negative Charged Protein-Polymer Conjugates”, Baker, S.L., Murata, H., Kaupbayeva, B., Tasbolat, A., Matyjaszewski, K., Russell, A.J., Biomacromolecules, 20, 2392-2405 (2019).

36. “Molecular Sieving on the Surface of a Nano-Armored Protein”, Kaupbayeva, B., Murata, H., Lucas, A., Matyjaszewski, K., Minden, J.S., Russell, A.J., Biomacromolecules, 20, 1235-1245 (2019).  

35. “Tailoring Site Specificity of Bioconjugation using Step-Wise ATRP on Proteins (SWAP)”, Carmali, S., Murata, H., Matyjaszewski, K., Russell, A.J., Biomacromolecules, 19, 4044-4051 (2018). 

34.  “Intramolecular biomimetic polymeric chaperones stabilize protein-polymer conjugates”, Baker, S., Munasinghe, A., Murata, H., Lin, P., Matyjaszewski, K., Colina, C.,Russell, A.J., Biomacromolecules, 19, 3798-3813 (2018).

33. “Solid-Phase Synthesis of Protein-Polymer on Reversible Immobilization Supports”, Murata, H., Carmali, S., Baker, S.L., Matyjaszewski, K., Russell, A.J., Nature Communications, 9, article number 845 (2018). 

32. “Bactericidal Specificity and Resistance Profile of Poly(Quaternary Ammonium) Polymers and Protein-Poly(Quaternary Ammonium) Conjugates”, Ji, W., Koepsel, R.R., Murata, H., Zadan, S., Campbell, A.S., Russell, A.J., Biomacromolecules, 18, 2583-2593 (2017).

31. “Tertiary Structure-Based Prediction of How ATRP Initiators react with Protein”, Carmali, S., Murata, H., Amemiya, E., Matyjaszewski, K., Russell, A.J., ACS Biomaterials Science and Engineering , 3, 2086-2097 (2017).

30. “ATRP-Grown Protein-Polymer Conjugates Containing Phenylpiperazine Selectively Enhance Transepithelial Protein Transport”, Cummings, C.S., Fein, K., Murata, H., Ball, R.L., Russell, A.J., Whitehead, K.A., J. Control. Release, 255, 270-278 (2017). 

29. “Polymer-Based Protein Engineering: Synthesis and Characterization of Armored, High Graft Density Polymer-Protein Conjugates”, Carmali, S., Murata, H., Cummings, C.S., Matyjaszewski, K., Russell, A.J., Method in Enzymology, 590, 347-380 (2017). 

28. “Design of Stomach Acid-Stable and Mucin-Binding Enzyme Polymer”, Cummings, C.S., Campbell, A.S., Baker, S.L., Carmali, S., Murata, H., Russell, A.J., Biomacromolecules, 18,   576-586 (2017). 

27. “Polymer-Based Protein Engineering Grown Ferrocene-Containing Redox Polymers Improve Current Generation in an Enzymatic Biofuel Cell”, Campbell, A.S., Murata, H., Carmali, S., Matyjaszewski, K., Islam, M.F., Russell, A.J.,  Biosens. Bioelectron., 86,   446-453 (2016). 

26. “The Effect of Covalently-Attached ATRP-Synthesized Polymers on Membrane Stability and Cytoprotection in Human Erythrocytes”, Clafshenkel, W.P., Murata, H., Anderson, J., Creeger, Y., Koepsel, R.R., Russell, A.J., PLOS ONE., 11, e015764 (2016). 

25. “Polymer-Based Protein Engineering Enable Molecular Dissolution of Chymotrypsin in Acetonitrile”, Cummings, C.S., Murata, H., Matyjaszewski, K., Russell, A.J., ACS Macro. Lett., 5, 493-497 (2016).

24. “Engineering of cell membranes with a bisphosphonate-containing polymer using ATRP   synthesis for bone targeting”, D’Souza, S., Murata, H., Jose, M.V.., Askarova, S., Yuntsen, Y., Andersen, J., Edington, C.D.J., Clafshenkel, W.P., Koepsel, R.R., Russell, A.J., Biomaterials, 35, 9447-9458 (2014).  

23. “Rational tailoring of substrate and inhibitor affinity via ATRP polymer-based protein engineering”, Murata, H., Cummings, C.S., Koepsel, R.R., Russell, A.J., Biomacromolecules, 15, 2817- 2823 (2014). 

22. “Dramatically increased pH and temperature stability of chymotrypsin using dual block polymer-based protein engineering”, Cummings, C.S., Murata, H., Koepsel, R.R., Russell, A.J., Biomacromolecules, 15, 763-771 (2014). 

21. “Multifunctional photo-crosslinked polymeric ionic hydrogel films”, He, H., Adzime, B., Zhong, M., Averick, S., Koepsel, R.R., Murata, H., Russell, A.J., Luebke, D., Takahara, A., Nulwala, H., Matyjaszewski, K., Polym. Chem., 5, 2824-2835 (2014). 

20. “Tailoring enzyme activity and stability using thermo responsive polymer-based protein engineering”, Cummings, C.S., Murata, H., Koepsel, R.R., Russell, A.J., Biomaterials, 34, 7473-7443 (2013). 

19. “Polymer-Based Protein Engineering Can Rationally Tune Enzyme Activity, pH-Dependence, and Stability”, Murata, H., Cummings, C.S., Koepsel, R.R., Russell, A.J., Biomacromolecules, 14, 1919-1926 (2013).

18. “Direct Electron Transfer in a Mediator-Free Glucose Oxidase-Based Carbon Nanotube Coated Biosensor”, Jose, M.V., Marx, S., Murata, H., Koepsel, R.R., Russell, A.J., Carbon, 50, 4010-4020 (2012). 

17. “Salicylic Acid-Releasing Polyurethane Acrylate Polymer as Anti-Biofilm Urological Catheter Coatings”, Nowatzki, P.J., Koepsel, R.R., Stoodley, P.,  Min, K.,  Harper, A., Murata, H.,  Donfack, J.,   Hortelano, E.R.,  Ehrlich, G.D.,  Russell, A.J., Acta Biomaterialia, 8, 1869-1880 (2012). 

16. “Tailoring the Trajectory of Cell Rolling with Designer Surfaces”, Edington, C., Murata, H., Koepsel, R.R., Andersen, J., Eom, S.E., Kanade, T., Balazs, A.,  Kolmakov, G., Kline, C., McKeel, D., Liron, Z., J. Russell, A.J., Langmuir, 27, 15345-15351 (2011).

15. “Simple Surface Modification of a Titanium Alloy with Silanated Zwitterionic Phosphorylcholine or Sulfobetaine Modifiers to Reduce Thrombogenicity”, Ye, S.H., Johnson Jr, C.A, Woolley, J.R., Murata, H., Gamble, L.J., Ishihara, K., Wagner, W.R., Colloids and Surfaces B: Biointerfaces, 79, 357-364 (2012). 

14. “Decontamination of Chemical and Biological Warfare Agents with a Single Multi-Functional Material”, Amitai, G., Murata, H., Andersen, J.D., Koepsel, R.R., Russell, A.J., Biomaterials, 31, 4417-4425 (2010).

13. “Non-Leaching Antibacterial Glass Surface via “Grafting onto”: the Effects of Number of QA on Biocidal Activity”, Huang, J., Koepsel, R.R., Murata, H., Wu, W., Lee, S.B., Dougherty, D., Kowalewski, T., Russell, A.J., Matyjaszewski, K., Langmuir, 24, 6785-6795 (2008).

12. “Permanent, Non-Leaching Antibacterial Surfaces 2.  How Cationic Surfaces Kill Bacterial Cells”, Murata, H., Koepsel, R.R., Matyjaszewski, K., Russell, A.J., Biomaterials, 28, 4870-4879 (2007). 

11. “Synthesis of Functionalized Polymer Monolayers from Active Ester Brushes”, Murata, H., Oswald Prucker, O., Rühe, J., Macromolecules, 40, 5497-5503 (2007).

10.  “Antibacterial Polypropylene via Surface-Initiated Atom Transfer Radical Polymerization”, Huang, J., Murata, H., Koepsel, R.R., Russell, A.J., Matyjaszewski, K., Biomacromolecules, 8, 1396-1399 (2007).

9. “Surface Active Antifungal Polyquaternary Amine”, Ravikumar, T., Murata, H., Koespel, R.R., Russell, A.J., Biomacromolecules, 7, 2762-2769 (2006). 

8. “Synthesis of Uniform Protein-Polymer Conjugates”, Lele, B.S., Murata, H., Matyjaszewski, K., Russell, A.J., Biomacromolecules, 6, 3380-3387 (2005). 

7. “Polymeric Coatings for Biomedical Devices”, Murata, H., Chang, B.J., Prucker, O., Dahm, M., Rühe, J., Surface Sci., 570, 111-118 (2004). 

6. “Novel Pathways for Tailor-Made Functional Coatings”, Prucker, O., Stöhr, T., Murata, H., Toomey, R., van Os, M.T., Förch, R., Knoll, W., Rühe, J., European Coating J., 40 (2003). 

5. “Synthesis and Radical Polymerization Behavior of Methacrylamides Having L-Leucyl-L-alanine Oligopeptide Moieties: Effect of the Peptide Chain Length on the Radical Polymerizability”, Murata, H., Sanda, F., Endo, T., Macromol. Chem. Phys., 202, 759-764 (2001).

4. “Synthesis and Radical Polymerization of a Novel Acrylamide Having an -Helical Peptide Structure in the Side Chain”, Murata, H., Sanda, F., Endo, T., J. Polym. Sci., Part A: Polym. Chem., 36, 1679 – 1682 (1998). 

3. “Synthesis and Radical Polymerization Behavior of a Novel Methacrylate Having L-Leucyl-L-alanyl-glycine Peptide Moiety”, Murata, H., Sanda, F., Endo, T., Macromol. Chem. Phys., 198, 2519 – 2529 (1997). 

2. “Synthesis and Radical Polymerization Behavior of Methacrylamides Having Peptide Moieties: Effect of the Methylene Chain Introduced between the Methacrylamide and Peptide Moieties on the Polymerizability and Polymerstructure”, Murata, H., Sanda, F., Endo, T., Macromolecules, 30, 2902 – 2906 (1997). 

1. “Highly Radical-Polymerizable Methacrylamide Having Dipeptide structure. Synthesis and Radical Polymerization of N-Methacryloyl-L-Leucyl-L-alanine Methyl Ester”, Murata, H., Sanda, F., Endo, T., Macromolecules, 29, 5535 – 5538 (1996).