Carnegie Mellon University

Ren Lab

Department of Biomedical Engineering



Klimas A, Gallagher BR, Wijesekara P, Fekir S, DiBernardo EF, Cheng Z, Stolz DB, Cambi F, Watkins SC, Brody SL, Horani A, Barth AL, Moore CI, Ren X, Zhao Y. Magnify is a universal molecular anchoring strategy for expansion microscopy. Nature Biotechnology. 2023.

Zhang Y, Wang F, Shi L, Lu M, Lee K-J, Ditty MM, Xing Y, He H-Z, Ren X, Zheng S-Y. Nanoscale coordination polymers enabling antioxidants inhibition for enhanced chemodynamic therapy. Journal of Controlled Release. 2023;354:196-206.


Wijesekara P*, Yadav P*, Perkins LA, Stolz D, Franks JM, Watkins S, Jacome ER, Brody SL, Horani A, Xu J, Barati Farimani A# and Ren X#. Engineering Rotating Apical-Out Airway Organoid for Assessing Respiratory Cilia Motility. iScience. 2022;104730

Ng WH, Johnston EK, Jie Tan J, Bliley JM, Feinberg AW, Stolz DB, Sun M, Wijesekara P, Hawkins F, Kotton DN and Ren X. Recapitulate Human Cardio-pulmonary Co-development Using Simultaneous Multilineage Differentiation of Pluripotent Stem Cells. eLife. 2022;11:e67872

Ng WH, Varghese B, Ren X. Co-differentiation and Co-maturation of Human Cardio-pulmonary Progenitors and Micro-Tissues from Human Induced Pluripotent Stem Cells. Bio-protocol. 2022;12:e4488.

Xing Y*, Yerneni SS*, Wang W, Taylor RE, Campbell PG# and Ren X#. Engineering pro-angiogenic biomaterials via chemoselective extracellular vesicle immobilization. Biomaterials. 2022;281:121357.

Hu M, Ling Z and Ren X. Extracellular Matrix Dynamics: Tracking in Biological Systems and Their Implications. Journal of Biological Engineering [Collection: Emerging leaders in biological engineering]. 2022; 16(1):13.

Shi L, Klimas A, Gallagher B, Cheng Z, Fu F, Wijesekara P, Miao Y, Ren X, Zhao Y and Min W. Super-resolution vibrational imaging using expansion stimulated Raman scattering microscopy. Advanced Science. 2022; 9(20):e2200315.

Varghese BC, Ling Z, and Ren X. Reconstructing the Pulmonary Niche with Stem Cells: A Lung Story. Stem Cell Research & Therapy. 2022; 13(1):1-12.

Petrosyan A, Montali F, Peloso A, Citro A, Byers LN, La Pointe C, Suleiman M, Marchetti A, Mcneill EP, Speer AL, Ng WH, Ren X, Bussolati B, Perin L, Di Nardo P, Cardinale V, Duisit J, Monetti AR, Savino JR, Asthana A, Orlando G. Regenerative medicine technologies applied to transplant update. Frontiers in Bioengineering and Biotechnology. 2022;10. doi: 10.3389/fbioe.2022.1015628


Klimas A, Gallagher B, Wijesekara P, Fekir S, Stolz D, Cambi F, Watkins S, Barth A, Moore C, Ren X and Zhao Y. Nanoscale Imaging of Biomolecules using Molecule Anchorable Gel-enabled Nanoscale In-situ Fluorescence Microscopy. Nature Portfolio. 2021:DOI: 10.21203/

Wijesekara P*, Liu Y*, Wang W, Johnston EK, Sullivan MLG, Taylor RE# and Ren X#. Accessing and Assessing the Cell-Surface Glycocalyx Using DNA Origami. Nano Letters. 2021;21:4765-4773. 

DeBari MK*, Ng WH*, Griffin MD, Kokai LE, Marra KG, Rubin JP, Ren X# and Abbott RD#. Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix. Biomimetics. 2021;6:52. 

Noda K, Philips BJ, Snyder ME, Phillippi JA, Sullivan M, Stolz DB, Ren X, Luketich JD and Sanchez PG. Heparanase inhibition preserves the endothelial glycocalyx in lung grafts and improves lung preservation and transplant outcomes. Scientific Reports. 2021;11:12265.

Xing Y*, Varghese B*, Ling Z, Kar A, Reinoso Jacome E, Ren X. Extracellular Matrix by Design: Native Biomaterial Fabrication and Functionalization to Boost Tissue Regeneration. Regenerative Engineering and Translational Medicine. 2021. 

Meng X*, Xing Y*, Li J*, Deng C, Li Y#, Ren X#, Zhang D#. Rebuilding the Vascular Network: In Vivo and In Vitro Approaches. Frontiers Cell and Developmental Biology. 2021;9:937. 

Speer AL, Ren X, McNeill EP, Aziz JM, Muir SM, Marino DI, Dadhich P, Sawant K, Ciccocioppo R, Asthana A, Bitar KN and Orlando G. Bioengineering of the digestive tract: approaching the clinic. Cytotherapy. 2021;23(5):381-389.

Liu Y*, Wijesekara P*, Kumar S, Wang W, Ren X# and Taylor R#. The Effects of Overhang Placement and Multivalency on Cell Labeling by DNA Origami. Nanoscale. 2021;13:6819-6828. 

Ling Z, Xing Y, Reinoso Jacome E, Fok SW and Ren X. Bioorthogonal Labeling and Chemoselective Functionalization of Lung Extracellular Matrix. Bio-protocol. 2021;11:e3922.


Wang W, Arias DS, Deserno M, Ren X and Taylor RE. Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics. APL Bioeng. 2020;4:041507.

Moser PT, Gerli M, Diercks GR, Evangelista-Leite D, Charest JM, Gershlak JR, Ren X, Gilpin SE, Jank BJ, Gaudette GR, Hartnick CJ and Ott HC. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue engineering Part A. 2020;26:543-555.


Comber EM, Palchesko RN, Ng WH, Ren X and Cook KE. De Novo Lung Biofabrication: Clinical Need, Construction Methods, and Design Strategy. Translational Research2019;211:1-18.

Citro A, Moser PT, Dugnani E, Rajab TK, Ren X, Evangelista-Leite D, Charest JM, Peloso A, Podesser BK, Manenti F, Pellegrini S, Piemonti L and Ott HC. Biofabrication of a vascularized islet organ for type 1 diabetes. Biomaterials. 2019;199:40-51.


Wijesekara P, Ng WH, Feng M and Ren X. Bioengineering the innate vasculature of complex organs: what have we learned so far. Current Opinion in Organ Transplantation. 2018;23:657-663.

Ren X, Evangelista-Leite D, Wu T, Rajab KT, Moser PT, Kitano K, Economopoulos KP, Gorman DE, Bloom JP, Tan JJ, Gilpin SE, Zhou H, Mathisen DJ and Ott HC. Metabolic glycan labeling and chemoselective functionalization of native biomaterials. Biomaterials. 2018;182:127-134.

Rajab TK, Okamoto T, Ren X, Mathisen DJ and Ott HC. Intralipid Improves Oxygenation after Orthotopic Lung Transplantation. The Journal of Heart and Lung Transplantation. 2018;38:225-227.

Zhou H, Kitano K, Ren X, Rajab TK, Wu M, Gilpin SE, Wu T, Baugh L, Black LD, Mathisen DJ and Ott HC. Bioengineering Human Lung Grafts on Porcine Matrix. Annals of Surgery. 2018;267:590-598.

2017 And Earlier

Kitano K, Schwartz DM, Zhou H, Gilpin SE, Wojtkiewicz GR, Ren X, Sommer CA, Capilla AV, Mathisen DJ,  Goldstein AM, Mostoslavsky G and Ott HC. Bioengineering of functional human induced pluripotent stem cell-derived intestinal grafts. Nature Communications. 2017;8:765.

Gilpin SE, Li Q, Evangelista-Leite D, Ren X, Reinhardt DP, Frey BL and Ott HC. Fibrillin-2 and Tenascin-C bridge the age gap in lung epithelial regeneration. Biomaterials. 2017;140:212-219.

Ren X. Invited commentary. Journal of Vascular Surgery. 2017;65:848.

Gilpin SE, Charest JM, Ren X, Tapias LF, Wu T, Evangelista-Leite D, Mathisen DJ and Ott HC. Regenerative potential of human airway stem cells in lung epithelial engineering. Biomaterials. 2016;108:111-119.

Gilpin SE, Charest JM, Ren X and Ott HC. Bioengineering Lungs for Transplantation. Thoracic Surgery Clinics. 2016;26:163-171.

Ren X, Moser PT, Gilpin SE, Okamoto T, Wu T, Tapias LF, Mercier FE, Xiong L, Ghawi R, Scadden DT, Ott HC: Engineering pulmonary vasculature in decellularized rat and human lungs. Nature Biotechnology 2015, 33(10):1097-102.

Tapias LF, Gilpin SE, Ren X, Wei L, Fuchs BC, Tanabe KK, Lanuti M, Ott HC: Assessment of Proliferation and Cytotoxicity in a Biomimetic Three-Dimensional Model of Lung Cancer. The Annals of Thoracic Surgery 2015, 100(2):414-421.

Ren X, Tapias LF, Jank BJ, Mathisen DJ, Lanuti M, Ott HC: Ex vivo non-invasive assessment of cell viability and proliferation in bio-engineered whole organ constructs. Biomaterials 2015, 52:103-112.

Jank BJ, Xiong L, Moser PT, Guyette JP, Ren X, Cetrulo CL, Leonard DA, Fernandez L, Fagan SP, Ott HC: Engineered composite tissue as a bioartificial limb graft. Biomaterials 2015, 61:246-256.

Ren X, Ott HC: On the road to bioartificial organs. Pflügers Archiv - European Journal of Physiology 2014, 466(10):1847-1857.

Gilpin SE, Ren X, Okamoto T, Guyette JP, Mou H, Rajagopal J, Mathisen DJ, Vacanti JP, Ott HC: Enhanced Lung Epithelial Specification of Human Induced Pluripotent Stem Cells on Decellularized Lung Matrix. The Annals of Thoracic Surgery, 2014, 98(5):1721-1729.

Guyette JP, Gilpin SE, Charest JM, Tapias LF, Ren X, Ott HC: Perfusion decellularization of whole organs. Nature Protocols 2014, 9(6):1451-1468.

Gilpin SE, Guyette JP, Gonzalez G, Ren X, Asara JM, Mathisen DJ, Vacanti JP, Ott HC: Perfusion decellularization of human and porcine lungs: Bringing the matrix to clinical scale. The Journal of Heart and Lung Transplantation 2013, 33(3):298-308.

Chen L, Ren X, Liang F, Li S, Zhong H, Lin S: Characterization of two novel small molecules targeting melanocyte development in zebrafish embryogenesis. Pigment Cell & Melanoma Research 2012, 25(4): 446-453.

Ren X, Gomez GA, Zhang B, Lin S: Scl isoforms act downstream of etsrp to specify angioblasts and definitive hematopoietic stem cells. Blood 2010, 115(26):5338-5346.

Wen L, Wei W, Gu W, Huang P, Ren X, Zhang Z, Zhu Z, Lin S, Zhang B: Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology 2008, 314(1):84-92.

Zhou C, Tai C, Ye HH, Ren X, Chen JG, Wang SQ and Chai Z. Interleukin‐1β downregulates the L‐type Ca2+ channel activity by depressing the expression of channel protein in cortical neurons. Journal of cellular physiology. 2006;206:799-806.

(* Equal contribution, # Co-correspondence)

Patent Applications

Ren X, Varghese BC, Kar AS, Napolitano JE, Xing YShape-Complementing, Porosity-Matching Perfusion Bioreactor System for Engineering Geometrically Complex Tissue Grafts. US Patent App. 17/478,202, 2022.

Ren X, Ott HC: Regeneration of a Functional Pulmonary Vascular Bed. US Patent, US10731135B2, 2020.

Ott HC, Ren X, Rajab KT, Bloom JP: Metabolic Labeling and Molecular Enhancement of Biological Materials Using Bioorthogonal Reactions. US Patent App. 15/624,399, 2017.