Resources & Equipment-The Center for Nucleic Acids Science and Technology - Carnegie Mellon University

Resources & Equipment

These CNAST or Mellon College of Science resources are available for general use by CNAST members.

ABI 2720 PCR Thermal Cycler

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

ABI 310 Genetic Analyzer Capillary Electrophoresis System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider
ABI 3130xl Genetic Analyzer Capillary Electrophoresis System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

ABI ViiA7 qPCR Machine

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider
Access to Modified DNA/RNA Sequences
  • For DNA up to 35 mers and RNA up to 30 mers
  • For incorporating modifications for which commercial specialty phosphoramidites are available (via GlenResearch)
Das Lab, MI 742
Contact: Subha Das
Agilent TapeStation
  • For rapid gel electrophoresis and automated
    analysis of RNA and DNA abundance, size, and integrity

McManus Lab, MI 255
Contact: Joel McManus

AKTA Explorerer FPLC Liquid-phase Chromatography System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Analytical and Semiprep HPLC Achim Lab, MI 835/839
Contact: Catalina Achim
ASI MS-2000 Programmable Translation Stage

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider
Beaglehole Variable-Angle Ellopsometry System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Beckman IC100 Murphy Lab Automation Suite, MBIC 1st floor
Contact: Robert Murphy

Beckman-Coulter P-ACE MDQ Automated Capillary Electrophoresis Systems

  • With UV, two-color LIF detection and photodiode array detection

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider
Biacore Surface Plasmon Resonance Instrument Das Lab, MI 742
Contact: Subha Das

Bio-rad Gel Dryers

  • Model 583
Das Lab, MI 742
Contact: Subha Das

Bio-Rad Protean Llxi Slab Gel Electrophoresis System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Bruker Nanoscope V MultiMode AFM System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Cary 300Bio UV-Vis Spectrophotometer

  • With temperature control

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Cary Eclipse Fluorescence Spectometer

  • With stopped-flow accessory, peltier multi-cell holder, and temperature control

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Cell Analyzer

  • 88/640 lasers with 96-well plate sipper attachment Intellicyte/Accuri C6
Cytometry Center, MBIC 1st floor
Contact: Yehuda Creeger

Cephied SmartCyclyer PCR workstation

  • With 16 sample chambers and 4 channel optical detection

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider
Circular Dichroism Center for Molecular Analysis, MI 555
Contact: Mark Bier
Coherent Optics QE Pro High-Performance Spectrometer

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider
Differential Scanning Calorimetry

Evilevitch Lab, WH 6409
Contact: Alex Evilevitch

Eppendorf 5415C Ultracentrifuge

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Eppendorf 5810R Ultracentrifuge

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Fluorescence Correlation Spectrometer

  • Zeiss Confocor 3 Unit
Microscopy Facility, MBIC 2nd Floor
Contact: Haibing Teng

Fluorescence Spectrometers

  • Cary Bio, Equipped for melting curve analysis (2)
  • Photon Technology Inc
Armitage Lab, MI 726
Contact: Bruce Armitage

Fluorescent Activated Cell Sorter

  • FACSVantage, 4 lasers (405, 488, 532, 640)
Cytometry Center, MBIC 1st floor
Contact: Yehuda Creeger
Forte Bio Red Evanescent Surface Sensor

Washburn Lab, MI 814
Contact: Newell Washburn

Gel Electrophoresis Equipment

  • For agarose and polyacrylamide gels - including large plates/gels – for gels 20cmx35cm
  • Reserved for use with nucleic acids (no proteins)
Das Lab, MI 742
Contact: Subha Das
Gel Filtration Chromatography Bruchez Lab Coldroom, MI 755
Contact: Marcel Bruchez

Gel/Culture Plate Scanner

  • Typhoon 9700

Location TBD (2nd floor currently)
Contact: Marcel Bruchez

Isothermal Titration Calorimetry

Rule Lab, MI 246
Contact: Gordon Rule

JASCO J-810 Circular Dichroism Spectrometer

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Jobin-Yvon Fluorolog-3 Florescence Spectrometer

  • With TIRF accessory

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

KSV 5000 Langmuir-Blodgett Trough

  • With dipping accessory, Wilhelmy plate, and surface potential accessory

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Labsmith Microflluidics Toolkit

  • Programmable DC power supply and chip prototypes

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Malvern Zatasizer Nano Dynamic Light Scattering System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Mass Spectrometer

  • Q-TOF-ESI/APCI/Nanospray
  • LCQ-ESI/APCI Ion Trap
  • MALDI
Center for Molecular Analysis, MI 555
Contact: Mark Bier
Mermade 4 DNA/RNA Synthesizer (Bioautomation) Das Lab, MI 742
Contact: Subha Das
Metamorph Imaging Software

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Nanodrop

  • Scanning UV-vis Spectrophotometer
  • For spectra with small sample (2-3 μL) volumes

Das Lab, MI 742 &
Shared Chemical Engineering Lab, DH 2203
Contact: Subha Das &

Jim Schneider

Nicolet Magna-IR 560 IR Spectrophotometer

  • With MCT and DTGS detection

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Nikon TE2000U Inverted Microscope

  • With high-resolution CCD camera (Photometrics Quatrum EM) and integrated laser system with interlocks

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

NMR Spectrometers Pittsburgh NMR Center for Biomedical Research,
MI 1st Floor
Apply To Use Facilities
Ocean Optics QE Pro High-Performance Spectrometer

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Perkin Elmer Spectrum CT Imaging System & Surgical Suite

  • IVIS/CT Small animal fluorescent/luminescent/CT imaging instrument
MI Vivarium

Point-Scanning Spectral Confocal Microscopes

  • Zeiss 510 Meta 2x
Shared Imaging Facility, MBIC 2nd floor
Contact: Haibing Teng
Potentiostat for Electrochemistry Measurements Achim Lab, MI 835/839
Contact: Catalina Achim
Preparative Ultracentrifuge MBIC Common Equipment Rm, 2nd floor
Contact: Haibing Teng

Spectral Fluorescence Plate Reader

  • Tecan Safire 2
MBIC, 2nd floor
Contact: Haibing Teng

Spectral Fluorescence/Luminescence Plate Reader with Anisotropy

  • Tecan M1000
Bruchez Lab, MI 755
Contact: Marcel Bruchez

SpectraMax M2 UV-Vis Fluorescence Microtiter Plate Reader System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider
Spinning Disk Confocal Microscope with Live-Cell Apparatus Shared Imaging Facility, MBIC 2nd floor
Contact: Haibing Teng
Stopped flow UV Vis Achim Lab, MI 835/839
Contact: Catalina Achim
Synergy H1 Hybrid Plate Reader System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider
Teledyne Isco Comniflash Rf 200i Flash Chromatography System

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Tokai Hit Transparent Thermoplate Stage

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

UV Vis Spectrometers

  • Cary 200
  • Cary 50
  • Cary 300, Equipped for melting curve analysis (2)
Armitage Lab, MI 726
Contact: Bruce Armitage

UVP Bio-DocIt Transilluminator

  • With CCD Image Capture

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Waters Alliance 2690 Analytic HPLC System

  • With heated column, PDA, fluorescence, and DLS detection

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider

Waters Delta 600 Semi-prep HPLC System

  • With PDA detection and fraction collector

Shared Chemical Engineering Lab, DH 2203

Contact: Jim Schneider