Subha R. Das-The Center for Nucleic Acids Science and Technology - Carnegie Mellon University

Subha R. Das

Associate Professor of Chemistry

Phone: (412) 268-6871

Research

Projects in the Das Lab are centered on nucleic acids chemistry. We have developed conditions for click-chemistry for the rapid modification and functionalization of DNA and RNA. With these we can rapidly get branched nucleic acids. Branched RNAs are mimics and probes for biologically important regulatory RNAs and branched DNAs are opening up new avenues in nanotechnology. Additionally we are exploring nucleic acid polymer hybrids as novel materials and delivery agents for RNA based therapeutics.

Projects in the Das lab are multi-disciplinary and reflect collaborative efforts with the labs of Mark Macbeth (Carnegie Mellon University, Biological Sciences), Linda Peteanu, Bruce Armitage, Krzysztof Matyjaszewski (Carnegie Mellon University, Chemistry) and Vamsi Yadavalli (Virginia Commonwealth University, Chemical and Life Science Engineering).

Publications

Das, S. R. The Kitchen Chemistry Sessions : The Kitchen Chemistry Sessions: Palatable Chemistry through Molecular Gastronomy and Cuisine. In Using Food to Stimulate Interest in the Chemistry Classroom; Symox, K., Ed.; American Chemical Society: Washington, DC, 2013.

Cho, H. Y.; Averick, S. E.; Paredes, E.; Wegner, K.; Averick, A.; Jurga, S.; Das, S. R.; Matyjaszewski, K. Star Polymers with a Cationic Core Prepared by ATRP for Cellular Nucleic Acids Delivery. Biomacromolecules 2013, 14, 1262–1267.

Paredes, E.; Das, S. R. RNA Conjugations and Ligations for RNA Nanotechnology. In RNA Nanotechnology and Therapeutics; Guo, P.; Haque, F., Eds.; CRC Press, 2013; pp. 197–211.

Paredes, E.; Zhang, X.; Ghodke, H.; Yadavalli, V. K.; Das, S. R. Backbone-Branched DNA Building Blocks for Facile Angular Control in Nanostructures. ACS Nano 2013, 7, 3953–3961.

Averick, S. E.; Paredes, E.; Grahacharya, D.; Woodman, B. F.; Miyake-Stoner, S. J.; Mehl, R. A.; Matyjaszewski, K.; Das, S. R. A Protein–Polymer Hybrid Mediated By DNA. Langmuir 2012, 28, 1954–1958.

Averick, S. E.; Paredes, E.; Irastorza, A.; Shrivats, A. R.; Srinivasan, A.; Siegwart, D. J.; Magenau, A. J.; Cho, H. Y.; Hsu, E.; Averick, A. A. et al. Preparation of Cationic Nanogels for Nucleic Acid Delivery. Biomacromolecules 2012, 13, 3445–3449.

Dey, S. K.; Paredes, E.; Evans, M.; Das, S. R. The Diverse Active Sites in Splicing, Debranching, and MicroRNA Processing Around RNA Phosphodiester Bonds. In From Nucleic Acids Sequences to Molecular Medicine; Erdmann, V. A.; Barciszewski, J., Eds.; Springer Berlin Heidelberg, 2012; pp. 475–501.

Paredes, E.; Das, S. R. Optimization of Acetonitrile Co-solvent and Copper Stoichiometry for Pseudo-ligandless Click Chemistry with Nucleic Acids. Bioorg. Med. Chem. Lett. 2012, 22, 5313–5316.

Averick, S.; Paredes, E.; Li, W.; Matyjaszewski, K.; Das, S. R. Direct DNA Conjugation to Star Polymers for Controlled Reversible Assemblies. Bioconjugate Chem. 2011, 22, 2030–7.

Paredes, E.; Das, S. R. Click Chemistry for Rapid Labeling and Ligation of RNA. ChemBioChem 2011, 12, 125–131.

Paredes, E.; Evans, M.; Das, S. R. RNA Labeling, Conjugation and Ligation. Methods 2011, 54, 251–259.