Carnegie Mellon University
Biomolecular Engineering Lab

Biomolecular Engineering Lab

The Biomolecular Engineering Laboratory combines the material facilities and the research perspectives of the Dahl, Domach, Schneider, Przybycien, Tilton and Whitehead research groups.  The open, shared space format of the laboratory is designed to provide an intellectually stimulating environment that promotes collaborative learning and research among graduate students and among research groups. It contains state of the art equipment for molecular biology, cell culture, cell manipulation and characterization, biological product separation and purification, biomolecular manipulation and characterization, chemical synthesis, biointerfacial characterization and active substance formulation and delivery. This laboratory is used, for example, to develop novel protein chromatography media, to improve the bio-efficacy of proteins undergoing sustained release drug delivery, to design unique surfactants that recognize and separate DNA in a sequence-specific manner, to develop novel responsive surfaces with thermally-programmed protein binding affinities, to design bioMEMS devices for cell separations, and to engineer state-of-the-art nanoparticles that can specifically deliver drugs to cancer cells.

The Biomolecular Engineering Laboratory provides the following major equipment capabilities:

Molecular Biology Tools

PCR workstation, nucleic acid and protein gel electrophoresis, gel imaging, plate reader

Cell Manipulation and Characterization Tools

Shaker flask through 10L fermenter, CO2 incubators, flow cytometer, centrifuges, epifluorescence microscopy

Biomolecular Spectroscopy Tools

UV-VIS, infrared, fluorescence, Raman, circular dichroism spectrometers

Analytical Separations Tools

Capillary electrophoresis, high pressure liquid chromatography, flash chromatography

Preparative Separations Tools

Low pressure chromatography, diafiltration and micro/ultrafiltration

Surface Preparation and Analysis Tools

Langmuir-Blodgett trough, surface plasmon resonance instrument, quartz crystal microbalance, spin-coater, atomic force microscope, ellipsometer