Sandra J. Kuhlman
159B Mellon Institute
Department of Biological Sciences
Carnegie Mellon University
4400 Fifth Avenue
Pittsburgh, PA 15213
Ph.D., University of Kentucky
Postdoctoral Appointments, Cold Spring Harbor laboratory and University of California, Los Angeles
How to build a brain for learning?
As adults we constantly face challenges in our environment and must adapt our brain circuits to deal with new situations. By splicing the DNA that codes for fluorescent proteins found in jellyfish and corals into the genome of mice, in combination with state-of-the-art multiphoton laser-scanning microscopy, we visualize and track the circuit changes that occur in the brains of awake behaving animals as they learn new skills.
How to build a circuit for learning in the first place?
The process of circuit assembly itself is finalized during the transition from childhood to adolescence, and influenced by experience. Construction must proceed correctly for further adult learning to occur. By comparing and contrasting learning in the young versus adult, we hope to identify, and ultimately rescue, the essential aspects of circuit construction that are vulnerable to deprivation and disease.
Feese BD, Pafundo DE, Schmehl MN, Kuhlman SJ. Binocular deprivation induces both age-dependent and age-independent forms of plasticity in parvalbumin inhibitory neuron visual response properties. J Neurophysiol. 2018 Feb 1;119(2):738-751. doi: 10.1152/jn.00386.2017. Epub 2017 Nov 8. PMID: 29118195
Samonds JM, Feese BD, Lee TS, Kuhlman SJ. Nonuniform surround suppression of visual responses in mouse V1. J Neurophysiol. 2017 Dec 1;118(6):3282-3292. doi:10.1152/jn.00172.2017. Epub 2017 Sep 20. PMID: 28931608
Jeon BB, Kuhlman SJ. The underdog pathway gets a boost. Nat Neurosci. 2017 Dec;20(12):1655-1656. doi: 10.1038/s41593-017-0019-7. PMID: 29184213
Pafundo DE, Nicholas MA, Zhang R, Kuhlman SJ. Top-Down-Mediated Facilitation in the Visual Cortex Is Gated by Subcortical Neuromodulation. J Neurosci. 2016 Mar 9;36(10):2904-14
Kuhlman SJ, O'Connor DH, Fox K, Svoboda K. Structural plasticity within the barrel cortex during initial phases of whisker-dependent learning. J Neurosci. 2014 Apr 23; 34(17):6078-83.
Kuhlman SJ, Olivas ND, Tring E, Ikrar T, Xu X, Trachtenberg JT. A disinhibitory microcircuit initiates critical-period plasticity in the visual cortex. Nature. 2013 Sep 26;501(7468):543-6. doi: 10.1038/nature12485.
Kuhlman SJ, Tring E, Trachtenberg JT. Fast-spiking interneurons have an initial orientation bias that is lost with vision. Nat Neurosci. 2011 Jul 12;14(9):1121-3. doi: 10.1038/nn.2890.
Kuhlman SJ, Lu J, Lazarus MS, Huang ZJ. Maturation of GABAergic inhibition promotes strengthening of temporally coherent inputs among convergent pathways. PLoS Comput Biol. 2010 Jun 3;6(6):e1000797.
Huang ZJ, Taniguchi H, He M, and Kuhlman S. Genetic labeling of neurons in mouse brain, in Imaging in Developmental Biology: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2010; p. 199.
Kuhlman SJ, Huang ZJ. High-resolution labeling and functional manipulation of specific neuron types in mouse brain by Cre-activated viral gene expression. PLoS One. 2008 Apr 16;3(4):e2005.
Kuhlman SJ and McMahon DG. Encoding the ins and outs of circadian pacemaking. Journal of Biological Rhythms. 2006; 21:470-81.