Stephanie Tristram-Nagle-Dept of Physics - Carnegie Mellon University

Stephanie Tristram-Nagle

Research Professor, Physics

Office: Wean Hall 6415
Phone: 412-268-3174
Fax: 412-681-0648


Ph.D., University of California, Berkeley


My research interests are in lipid membrane structure, properties and thermodynamics. Our Biophysics laboratory explores the structure of biological phospholipids, lipid/peptide and lipid/cholesterol mixtures using oriented samples on substrates and unoriented multilamellar or large unilamellar vesicles using X-ray diffuse scattering with a Rigaku rotating anode and CCD detector here at CMU, and with synchrotron radiation at the Cornell High Energy Synchrotron Source.  The X-ray data yield not only structure, such as lipid area and membrane thickness, but also the bending and compression moduli of the membranes. I collaborate with molecular dynamics simulators to compare our structural results with simulated results. The second technique which yields lipid volume as a function of temperature is density equilibration, using D2O-H2O mixtures.  We also collect volume scans vs temperature with an Anton-Paar DMA 5000 M densimeter.

Selected Publications

  • J. F. Nagle, M. Jablin, S. Tristram-Nagle, Sugar Does Not Affect the Bending and Tilt Moduli of Simple Lipid Bilayers. Chem. Phys. Lipids 196, 76 (2016)
  • J. F. Nagle, K. Akabori, B. W. Treece, S. Tristram-Nagle, Determination of Mosaicity in Oriented Stacks of Lipid Bilayers, Soft Matter 12, 1884 (2016)
  • S. Tristram-Nagle, Use of X-Ray and Neutron Scattering Methods with Volume Measurements to Determine Lipid Bilayer Structure and Number of Water Molecules/Lipid. In Membrane Hydration, Subcellular Biochemistry, Vol. 71, pp. 17-43. Ed. E. A. Disalvo, Springer (2015)
  • Chris Neale, Kun Huang, Angel García, Stephanie Tristram-Nagle, Penetration of HIV-1 Tat47–57 into PC/PE Bilayers Assessed by MD Simulation and X-ray Scattering, Membranes 5, 473 (2015)
  • Yunyan Qiu, Jacob Mohin, Chia-Hua Tsai, Stephanie Tristram-Nagle, Roberto R. Gil, Tomasz Kowalewski, Kevin J. T. Noonan, Stille Catalyst-Transfer Polycondensation Using Pd-PEPPSI-IPr for High-Molecular-Weight Regioregular Poly(3-hexylthiophene), Macromolecular Rapid Communications 36, 840 (2015)
  • Kiyotaka Akabori, Kun Huang, Bradley W. Treece, Michael S. Jablin, Brian Maranville, Arthur Woll, John F. Nagle, Angel E. Garcia, Stephanie Tristram-Nagle, HIV-1 Tat membrane interactions probed using X-ray and neutron scattering, CD spectroscopy and MD simulations, Biochimica et Biophysica Acta (BBA) - Biomembranes 1838, 3078 (2014)
  • Alexander L. Boscia, Bradley W. Treece, Dariush Mohammadyani, Judith Klein-Seetharaman, Anthony R. Braun, Tsjerk A. Wassenaar, Beate Klösgen, Stephanie Tristram-Nagle, X-ray structure, thermodynamics, elastic properties and MD simulations of cardiolipin/dimyristoylphosphatidylcholine mixed membranes, Chemistry and Physics of Lipids 178, 1 (2014)
  • Alexander L. Boscia, Kiyotaka Akabori, Zachary Benamram, Jonathan A. Michel, Michael S. Jablin, Jonathan D. Steckbeck, Ronald C. Montelaro, John F. Nagle, Stephanie Tristram-Nagle, Membrane Structure Correlates to Function of LLP2 on the Cytoplasmic Tail of HIV-1 gp41 Protein, Biophysical Journal 105, 657 (2013)
  • Kelsey M. Hallinen, Stephanie Tristram-Nagle, John F. Nagle, Volumetric stability of lipid bilayers, Physical Chemistry Chemical Physics 14, 15452 (2012)
  • Jianjun Pan, Frederick A. Heberle, Stephanie Tristram-Nagle, Michelle Szymanski, Mary Koepfinger, John Katsaras, Norbert Kučerka, Molecular structures of fluid phase phosphatidylglycerol bilayers as determined by small angle neutron and X-ray scattering, Biochimica et Biophysica Acta (BBA) - Biomembranes 1818, 2135 (2012)
  • Mohit Raghunathan, Yuriy Zubovski, Richard M. Venable, Richard W. Pastor, John F. Nagle, Stephanie Tristram-Nagle, Structure and Elasticity of Lipid Membranes with Genistein and Daidzein Bioflavinoids Using X-ray Scattering and MD Simulations, The Journal of Physical Chemistry B 116, 3918 (2012)
  • Anthony R. Braun, Eva Sevcsik, Pamela Chin, Elizabeth Rhoades, Stephanie Tristram-Nagle, Jonathan N. Sachs, α-Synuclein Induces Both Positive Mean Curvature and Negative Gaussian Curvature in Membranes, Journal of the American Chemical Society 134, 2613 (2012)
  • P Shchelokovskyy, S Tristram-Nagle, R Dimova, Effect of the HIV-1 fusion peptide on the mechanical properties and leaflet coupling of lipid bilayers, New Journal of Physics 13, 025004 (2011)
  • Pradeep Uppamoochikkal, Stephanie Tristram-Nagle, John F. Nagle, Orientation of Tie-Lines in the Phase Diagram of DOPC/DPPC/Cholesterol Model Biomembranes, Langmuir 26, 17363 (2010)
  • Stephanie Tristram-Nagle, Rob Chan, Edgar Kooijman, Pradeep Uppamoochikkal, Wei Qiang, David P. Weliky, John F. Nagle, HIV Fusion Peptide Penetrates, Disorders, and Softens T-Cell Membrane Mimics, Journal of Molecular Biology 402, 139 (2010)
  • Jianjun Pan, Stephanie Tristram-Nagle, John Nagle, Effect of cholesterol on structural and mechanical properties of membranes depends on lipid chain saturation, Physical Review E 80, 021931 (2009)