PCS3 Trial Outline
The figure below illustrates the temporal sequence of Pittsburgh Cold Study 3 (PCS3) study procedures. See the table below for a more detailed description of the measures taken during each study phase.
Figure 1. Temporal sequence of PCS3 study activities. >>Download PowerPoint Slide<<
PCS3 Measures by Study Phase
| Study Phase | Measures Collected | |
| 10-12 weeks Pre-Quarantine |
• 10-ml blood draw: viral-specific serum Ab |
|
| 7-8 weeks Pre-Quarantine |
• 25-ml blood draw: o HIV; o Complete blood count (CBC & serum chemistry; o telomere length (1/3 sample) • Urine sample: urinalysis; pregnancy test • First day of last menstrual period (LMP) (#1) • Screening physical exam • Buccal scraping: genotyping • Saliva sample: cotinine • Hair sample: cortisol (#1) • Questionnaire Set 1 |
|
| 6 weeks Pre-Quarantine |
• 2-day repeated salivary cortisol collections (#1 & #2) • LMP (#2) • Questionnaire Set 2 |
|
| 5 weeks Pre-Quarantine |
• Begin 2-week daily interviews: o social interactions o affect o health practices • 4 days of pedometry: physical activity |
|
| 3-21 days Pre-Quarantine |
• Urine sample: pregnancy test • Laboratory acute stress reactivity #1 • Questionnaire Set 3 |
|
| 8-21 days Pre-Quarantine |
• 7 days of 24-hr actigraphy: energy expenditure/sleep |
|
| 3 to 5 Days Pre-Quarantine |
• Questionnaire Set 4 • 10-15-ml blood draw: viral specific Ab; telomere length (1/3 sample) |
|
| Quarantine Day 0 (Baseline) |
• Hair samples: cortisol (#2) • Questionnaire Sets 5, 6, 7, & 8 • 1-day repeated saliva cortisol collection (#3) • Urine sample: isoprostanes; pregnancy test • LMP (#3) • Spirometry • 15-ml blood draw: telomere length (1/3 sample) • Nasal wash: viral isolation • General physical exam • Ear, nose, and throat (ENT) exam • Upper respiratory illness (URI) symptoms (self-report) • Daily affect & health practices |
|
|
End Quarantine Day 0 |
Inoculation with Virus (i.e., Viral Challenge) |
|
| Quarantine Days 1 through 5 |
• Nasal wash: viral isolation & quantification; cytokines • URI indicators of pathophysiology • General physical exam • ENT exam • URI symptoms • Daily affect & health practices |
|
| End Quarantine Day 5 |
• Hair sample: cortisol (#3) • Exit physical exam |
|
| 4 weeks Post-Challenge |
• Urine pregnancy test • LMP (#4) • Laboratory acute stress reactivity (#2) • 10-ml blood draw: viral-specific serum Ab |
|
Study Questionnaire Sets
Screening Questionnaire
Alcohol Consumption QuestionnaireHistory and Current Status of Smoking
Paffenbarger Activity Questionnaire
The Pittsburgh Sleep Quality Index
Breakfast
Short Form Health Survey
Questionnaire Set 1
DemographicsObjective Socioeconomic Status
MacArthur Scale of Subjective Social Status
Parents' Age and Vital Status
Clark Marital Intimacy Scale
Social Network Index
Interpersonal Support Evaluation List
Social Convoy Circles
Trait Affect Scale (#1)
Perceived Stress Scale
Questionnaire Set 2
SC Social Participation MeasureSC Perceived Community Scale
Ryff Scales of Psychological Well-Being
Short Loneliness Scale
Negative Interaction Items
Tucker Social Control
Revised Life Orientation Test
Shyness Index
Questionnaire Set 3
IPIP Big-Five Factor Markers (Extroversion & Agreeableness only)Communal Orientation Scale
Life Events List
Laboratory Questionnaire
Stress Reactivity QuestionnaireRumination Measure
Emotional Response to Stress Questionnaire
Questionnaire Set 4
Giving Support - Interpersonal Support Evaluation ListOpener Scale
Adult Attachment Questionnaire
Positive and Negative Affect Schedule (items 1-30)
Toronto Alexithymia Scale
Questionnaire Set 5
SC Childhood InterviewChildhood Subjective Social Status
Questionnaire Set 6
Family Environment ScaleParental Bonding Instrument
Risky Families Questionnaire
Positive and Negative Affect Schedule (items 31-60)
Questionnaire Set 7
Parental Social ParticipationSC Places You’ve Lived Interview
Recalled Childhood and Adolescence Perceived Stress
Questionnaire Set 8
IPIP Big-Five Factor MarkersCook-Medley Hostility Scale
Trait Affect Scale (#2)
Emotion Regulation Questionnaire