Kris Noel Dahl-Chemical Engineering - Carnegie Mellon University

Kris Noel Dahl

Associate Professor of Chemical Engineering and BioMedical Engineering

Office: Doherty Hall 2100C
Phone: 412-268-9609
Fax: 412-268-7139

Bio

Prof. Kris N. Dahl joined Carnegie Mellon University in 2006. She received her B.S. in Chemical Engineering from Carnegie Mellon in 1998 and her Ph.D. in Chemical Engineering from the University of Pennsylvania in 2004. Her thesis research was on the mechanics and architecture of composite membrane systems under the supervision of Dennis Discher. Prof. Dahl then completed a postdoctoral appointment at the Johns Hopkins University School of Medicine in the Department of Cell Biology where she focused on the biochemical and functional analysis of spectrin-repeat complexes at the nuclear envelope under Katherine L. Wilson.

Education

B.S. in Chemical Engineering from Carnegie Mellon in 1998

Ph.D. in Chemical Engineering from the University of Pennsylvania in 2004

Research

Professor Dahl uses rheological, biophysical and optical techniques to understand the structure and organization of the cell nucleus. These studies are relevant to dissecting the molecular pathology of diseases caused by defects in nuclear structure.

Diseases of the nuclear lamina.

Many diseases result from the loss or mutation of lamins and other structural proteins at the nuclear envelope and in the nuclear interior. Diseases include Hutchinson-Gilford Progeria Syndrome (premature aging), Emery-Dreifuss Muscular Dystrophy and Dilated Cardiomyopathy. Nuclear mechanical integrity is significantly altered in some cells of these patients. Professor Dahl's studies compare normal cells with cells in which structural proteins are either chronically absent (as in knockout animals or disease patient cells) or rapidly down-regulated (as by RNAi-mediated gene silencing). These studies reveal the adaptations made by nuclei to altered mechano-structural environments in order to restore function.

Stem cell differentiation and cancer progression.

Nuclear shape and chromosome positioning change dramatically during stem cell differentiation and cancer progression. However, these changes have not been quantified and their downstream effects are poorly understood. Fluorescence techniques are being combined with quantitative biophysics to track recruitment of transcription factors or cell cycle regulators to differentiationspecific or cancer-specific genes while cells or nuclei are under well-defined imposed forces.

Mechanotransduction

Mechanotransduction allows cells to sense mechanical forces and adapt by changing gene expression. Signal transduction to the nucleus plays a significant role in gene expression, but mechanical forces may also propagate through the cell to the nucleus. Combining molecular biology and mechanical measurements with computational continuum mechanical modeling allows determination of the range of forces that can be transduced into the nucleus. Simultaneously, molecular force-induced changes in gene expression can be examined for any system of interest. These studies are central to tissue and cellular engineering to provide cells in an artificial environment with the correct mechanical information.

Research Websites

Biomolecular Engineering
Research Group Site

Highlights

  • Co-organized five annual Biomechanics Day symposia for the Pittsburgh area.
  • Member (and chair 2013) of the university laboratory safety committee
  • Primary contributing author for online course in Anatomy and Physiology (through OLI and Acrobatiq)

Awards and Honors

  • Invited speaker at The National Cancer Institute's Physical Sciences-Oncology Symposium, 2016
  • National Science Foundation CAREER Award 2010
  • Kun Li Award for excellence in education, awarded by graduating seniors of Chemical Engineering
  • Ruth L. Kirschstein National Research Service Award: Post-doctoral research fellowship from the NIH
  • American Society of Cell Biology 2004 annual society meeting Press Book as one of twelve of the most "newsworthy, significant or highly intriguing" research projects of the year
  • Whitaker Graduate Fellowship: Graduate research fellowship for biotechnology research

   

Publications

Recent Publications
Selected Publications
Full Publications

Recent Publications

Shams H, Holt BD, Mahboobi SH, Jahed Z, Islam MF, Dahl KN, Mofrad MR (2014) Actin reorganization through dynamic interactions with single-wall carbon nanotubes. ACS Nano. [Epub ahead of print]

Kalinowski A, Qin Z, Coffey K, Kodali R, Buehler MJ, Lösche M, Dahl KN (2013) Calcium Causes a Conformational Change in Lamin A Tail Domain that Promotes Farnesyl-Mediated Membrane Association. Biophysical Journal 104(10):2246-53

Booth-Gauthier EA, Du V, Ghibaudo M, Rape AD, Dahl KN, Ladoux B (2013) Hutchinson-Gilford progeria syndrome alters nuclear shape and reduces cell motility in three dimensional model substrates. Integrative Biology 5(3) 569-77

Booth-Gauthier EA, Alcoser TA, Yang G, Dahl KN (2012) Force induced changes in subnuclear movement and rheology. Biophysical Journal 103 (12): 2423-31

Boyer PD, Holt BD, Islam MF, Dahl KN (2012) Decoding membrane- versus receptor-mediated delivery of single-walled carbon nanotubes into macrophages using modifications of nanotube surface coatings and cell activity. Soft Matter 9: 758-764    

Selected Publications

Kalinowski A, Qin Z, Coffey K, Kodali R, Buehler MJ, Lösche M, Dahl KN (2013) Calcium Causes a Conformational Change in Lamin A Tail Domain that Promotes Farnesyl-Mediated Membrane Association. Biophysical Journal 104(10):2246-53

Booth-Gauthier EA, Alcoser TA, Yang G, Dahl KN (2012) Force induced changes in subnuclear movement and rheology. Biophysical Journal 103 (12): 2423-31

Ribeiro AJS, Tottey S, Taylor RWE, Bise R, Kanade T, Badylak SF, Dahl KN (2012) Mechanical characterization of adult stem cells from bone marrow and perivascular niches. Journal of Biomechanics 45(7):1280-7

Zhong Z, Booth-Gauthier EA, Dahl KN (2011) ?II spectrin stabilizes stress fibers and actin-membrane interactions. Cellular and Molecular Bioengineering 4(1):106-115


Full Publications

26. Shams H, Holt BD, Mahboobi SH, Jahed Z, Islam MF, Dahl KN, Mofrad MR (2014) Actin reorganization through dynamic interactions with single-wall carbon nanotubes. ACS Nano. [Epub ahead of print]

25. Kalinowski A, Qin Z, Coffey K, Kodali R, Buehler MJ, Lösche M, Dahl KN (2013) Calcium Causes a Conformational Change in Lamin A Tail Domain that Promotes Farnesyl-Mediated Membrane Association. Biophysical Journal 104(10):2246-53

24. Booth-Gauthier EA, Du V, Ghibaudo M, Rape AD, Dahl KN, Ladoux B (2013) Hutchinson-Gilford progeria syndrome alters nuclear shape and reduces cell motility in three dimensional model substrates. Integrative Biology 5(3) 569-77

23. Booth-Gauthier EA, Alcoser TA, Yang G, Dahl KN (2012) Force induced changes in subnuclear movement and rheology. Biophysical Journal 103 (12): 2423-31

22. Boyer PD, Holt BD, Islam MF, Dahl KN (2012) Decoding membrane- versus receptor-mediated delivery of single-walled carbon nanotubes into macrophages using modifications of nanotube surface coatings and cell activity. Soft Matter 9: 758-764    

21. Holt BD, McCorry MC, Boyer PD, Dahl KN, Islam MF (2012) Not all protein-mediated single-wall carbon nanotube dispersions are equally bioactive. Nanoscale 4(23): 7425-34

20. Holt BD, Shams H, Horst TA, Basu S, Rape AD, Wang Y-L, Rohde GK, Mofrad M, Islam MF, Dahl KN (2012) Altered cell mechanics from the inside: dispersed single wall carbon nanotubes integrate with and restructure actin. Journal of Functional Biomaterials 3(20): 298-417

19. Holt BD, Dahl KN, Islam MF (2012) Cells take up and recover from protein-stabilized single-wall carbon nanotubes with two distinct rates. ACS Nano 6(4):3481-90

18. Ribeiro AJS, Tottey S, Taylor RWE, Bise R, Kanade T, Badylak SF, Dahl KN (2012) Mechanical characterization of adult stem cells from bone marrow and perivascular niches. Journal of Biomechanics 45(7):1280-7

17. Wren NJ, Zhong Z, Schwartz R, Dahl KN (2012) Modeling nuclear blebs in a nucleoskeleton of independent filament networks. Cellular and Molecular Bioengineering 5(1):73-81

16. Choi S, Wang W, Ribeiro AJS, Kalinowski A, Gregg A, Opreska P, Niedernhofer LJ, Rohde GK, Dahl KN (2011) Multi-space computational image analysis of nuclear morphology associated with various nuclear-specific aging disorders. Nucleus  2(6):570-9

15. Yaron PN, Holt BD, Short PA, Losche PM, Islam MF, Dahl KN (2011) Single wall carbon nanotube uptake into cells by endocytosis not membrane penetration. Journal of Nanobiotechnology 9:45

14. Qin Z, Kalinowski A, Dahl KN, Buehler MJ (2011) Structure and stability of the lamin A tail domain and HGPS mutant. Journal of Structural Biology 175(3): 425-33

13. Holt BD, Dahl KN, Islam MF (2011) Quantification of uptake and localization of bovine serum albumin-stabilized single-wall carbon nanotubes in different human cell types. Small 7(16): 2348-55

12. Zhong Z, Booth-Gauthier EA, Dahl KN (2011) ?II spectrin stabilizes stress fibers and actin-membrane interactions. Cellular and Molecular Bioengineering 4(1):106-115

11. Holt BD, Short PA, Rape AD, Wang YL, Islam MF, Dahl KN (2010) Carbon nanotubes reorganize actin structures in cells and ex vivo. ACS Nano 4(8):4872-8

10. Zhong Z, Chang SA, Kalinowski A, Wilson KL, Dahl KN (2010) Stabilization of the spectrin-like domains of nesprin-1alpha by the evolutionarily conserved "adaptive" domain. Cellular and Molecular Bioengineering 3(2):139-150   

9. Philip JT and Dahl KN(2008) Nuclear mechanotransduction: response of the lamina to extracellular stress with implications in aging. Journal of Biomechanics 41(15):3164-70

8. Pajerowski JD, Dahl KN, Zhong FL, Sammak PJ, Discher DE (2007) Physical plasticity of the nucleus in stem cell differentiation. Proceedings of the National Academy of Science USA; 104:15619-24

7. Dahl KN, Scaffidi P, Islam MF, Yodh AG, Wilson KL, Misteli T (2006) Distinct structural and mechanical properties of the nuclear lamina in Hutchinson-Gilford progeria syndrome. Proceedings of the National Academy of Science USA; 103(27):10271-6

6. Subramanian S, Tsai R, Sen S, Dahl KN, Discher DE (2006) Membrane mobility and clustering of Integrin Associated Protein (IAP, CD47)-Major differences between mouse and man and implications for signaling. Blood Cells, Molecules and Diseases; 36(3):364-72

5. Dahl KN, Engler AJ, Pajerowski JD and Discher DE (2005) Power-law rheology of isolated nuclei with deformation mapping of nuclear sub-structures. Biophysical Journal; 89: 2855-2864

4. Dahl KN, Kahn SM, Wilson KL and Discher DE (2004) The nuclear envelope lamina network has elasticity and incompressibility suggestive of a molecular shock absorber. Journal of Cell Science; 117:4779-4786 with Editor’s Highlight and Research highlight in October 7, 2004 issue of Nature

3. Dahl KN, Parthasarathy R, Westhoff CM, Layton DM and Discher DE (2004) Protein 4.2 is critical to the CD47-membrane skeleton attachment in the human red cell. Blood 2004; 103:1131-1136

2. Dahl KN, Westhoff CM, and Discher DE (2003) Fractional attachment of CD47 (IAP) to the erythrocyte cytoskeleton and visual co-localization with Rh protein complexes. Blood; 101:1194-1199

1. Subramanian A, Ma H, Dahl KN, Zhu J and Diamond SL (2002) Adenovirus or HA-2 fusogenic peptide-assisted lipofection increases cytoplasmic levels of plasmid in nondividing endothelium with little enhancement of transgene expression. The Journal of Gene Medicine; 4: 75-83