Spring 2011 Journal Club (Graduate Research Seminar)-Department of Biological Sciences - Carnegie Mellon University

Spring 2011 Journal Club

(Graduate Research Seminar)

Journal club is held in the Conference Room of the Mellon Institute at 12:30 p.m. on Wednesdays unless otherwise indicated.

Date

Speaker

Topic

1/12 Brenna McCauley
Evolution of a Novel Cell Population in Sea Urchin Embryos: Changes in Specification and Cell Fate
1/19 Jeanne Morin-Leisk
Mutational Analysis of a Large GTPase Required for ER Network Formation

Kalin Vasilev
Biosensors for Protein Proximity at the Cell Surface
1/26 Anmol Grover Understanding Translation at Single Molecule Level

Alan Waggoner, Ph.D.
Optogenetics
2/2 Smita Yadav
At Long Last, a Golgi Receptor Complex for the Dynein Motor!

Qi Yan
Super-resolution Imaging Using Fluorogen Activating Proteins
(FAPs)
2/8
(2-4 p.m.)
Rotation 2 Presentations

Karen Kormuth
Peptide Nucleic Acids (PNAs) as Inhibitors of Nucleolin/rDNA G-quadruplex Binding
Advisor: Armitage

Sahil Sangani
Investigating the Role of Kinesin Head Domain Surface Residues in Tail Domain Binding
Advisor: Hackney

John Pettersson
Translation in a Crowded Environment
Advisor: Bruchez

Jigarkumar Desai
PNAs: Potential Probes for Site-specific Labeling of Eukaryotic Ribosomes
Advisor: Bruchez

Shanna Bowersox
Agonist Stimulated Internalization of a FAP-Tagged µ-Opioid Receptor
Advisor: Jarvik

Eugenio Gallo
Using Biosensors for Detecting Protein Proximity at the Cell Surface
Advisor: Jarvik

Madhumitha Ramesh
Investigating the Role of RhoB in Actin-mediated b2AR Recycling
Advisor: Puthenveedu

2/9
(12:30-2 p.m.)
Rotation 2 Presentations

Ting Liu
Study of hADAR2 Catalytic Domain Mutants – How Substrate Specificity Information is Hidden in the Structure
Advisor: Macbeth

Elizabeth Ransey
Purification, Crystallization and Mechanistic Studies of RNA Lariat De-branching Enzyme,DBR1
Advisor: Macbeth

Salini Konikkat
Cell-cycle Dependent Phosphorylation of APC2 in Drosophila Embryos
Advisor: McCartney

Zhongling Sun
Spatial and Temporal Expression of alx1-like in the Sea Urchin Embryo
Advisor: McCartney

Han Lai
Protein Location and Sorting Pathway: A Hidden Markov Approach
Advisor: Murphy

Yi Wang
Application of Lanthanide Ions on NMR Analysis of EcoRV
Advisor: Rule

Olivia Molinar
Characterization of Axonal Transport Using High Resolution Imaging Methods
Advisor: Yang

2/16 Bradley Yates Progress Toward Creation of an scFv-based Protein-Protein Interaction Biosensor

Kaitlyn Dykstra
How Does Yip1A Structure the Mammalian Endoplasmic Reticulum?
2/23
Emily Furbee
Designing a Better in vivo Autophagy Reporter

Nathan Urban, Ph.D.
A Dimorphic Pheromone Circuit in Drosophila from Sensory Input to Descending Output

Ruta V, Datta SR, Vasconcelos ML, Freeland J, Looger LL, Axel R. Nature 468, 686–690 (02 December 2010). doi:10.1038/nature09554

3/2
Ashrifia Adomako-Ankomah Regulation of Skeletal Morphogenesis in the Sea Urchin Embyro

Jing Wen Input Specific Critical Periods for Experience-dependent Plasticity in Layer 2/3 Pyramidal Neurons
3/9 No Journal Club - Spring Break
3/16 Timothy Jarvela
Understanding the Dynamics of the Golgi Through Acute Inactivation

Veronica Hinman, Ph.D.
Phenotypic Robustness Conferred by Apparently Redundant Transcriptional Enhancers
Frankel N, Davis GK, Vargas D, Wang S, Payre F, Stern DL. Nature. 466(7305):490-3, 2010 Jul 22.
3/23 Merissa Remus Region-specific ERK Activation in the Nucleus Accumbens in Response to Reward-predictive Cues: Does Behavioral Context Matter?
Javier Lopez, Ph.D.
Is Aging Necessary? Sexual Antagonism, Epistasis and Deleterious Pleiotropy of Lifespan-extending Mutations in Drosophila
Maguire et al. Quantitative and molecular genetic analyses of mutations increasing Drosophila life span. PLoS Genetics 6:e1001037, 2010.
3/30 Ayshwarya Subramanian Inferring Evolution of Cell Lineages in Breast Cancer

Jesse Sheehan

Impact of Global and Local Attention on Neuronal Activity in Inferotemporal Cortex

Perceptual psychologists have made frequent use of hierarchical displays to analyze global and local visual perception in humans.  Hierarchical displays are well suited to this purpose because they allow measuring responses to the same shape both at the level of the whole object and at the level of its parts.  A key phenomenon discovered by the use of such stimuli is the global advantage effect:  humans report global shape faster then local shape.  Inferotemporal cortex (IT), as the terminus of the ventral stream, a chain of areas dedicated to pattern vision, contains neurons selective for complex images.  Recent work in the laboratory has shown that IT neurons in the macaque monkey exhibit a signal for global information ~30ms sooner than local information while passively viewing hierarchical displays.  

My research aims to expound upon this finding by requiring the active processing of the global and local content of the displays.  Monkeys are cued which level (global or local) of the display to attend to, and must respond with the identity (shape) of the object at that level.  This will attempt to answer two main questions:  Do monkeys exhibit a behavioral global advantage effect while performing such a task?  Is the discriminative signal latency in IT neurons the same during active as during passive processing?

4/6 Mark DeBlois Continuous Sensory Input is Not Required to Maintain Synaptic Strength after Plasticity Onset in the Mouse Barrel Cortex

Jason Talkish
Structuring the Preribosome One Subcomplex at a Time: in vivo RNA Footprinting Reveals the RNA Binding Function of Nop12
4/13 Santosh Chandrasekaran Exploring the Role of Diversity in Cortical Neural Circuits

Jineta Banerjee
Knocking out GABAA Receptor in the Mouse Auditory Brainstem in a Tissue Specific Manner
4/20
Kiran Rafiq
Linking a Developmental Gene Regulatory Network to Morphogenesis

Alys Cheatle
Subtle Changes in DNA Binding May Explain Drastic Rewiring of Tbr Target Genes during Echinoderm Evolution
4/27 David Whitney
Evoking Neural Plasticity in the Visual Cortex Depends on Local Map Structure

Cassandra Priddy
The Role of Ykt6 in Golgi Membrane Fusion Events
5/4 (Mellon Institute Social Room, 12:30 - 1:30 p.m.)
Rotation 3 Presentations

Yi Wang
The Role of GMAP210 and G160 Interaction in Golgi Positioning
Advisor: Linstedt

Sahil Sangani
Cloning, Expression and Purification of scDBR1 for Mechanistic and Crystallographic Studies
Advisor: Macbeth

Jigarkumar Desai
Crystal Structure of hADAR2 Catalytic Domain Mutant: Unraveling the Mechanism behind Substrate Specificity
Advisor: Macbeth

Shanna Bowersox
Effects of an Inflammatory Environment on Gene Expression of BMP2-induced Osteoblastic Differentiation of C2C12 Progenitor Cells
Advisor: Campbell

Elizabeth Ransey
The Effects of Inflammation on Muscle-Derived Stem Cell (MDSC) Differentiation: Implications for Stem-Cell Therapy
Advisor: Campbell

5/5 (Mellon Institute Social Room, 10 - 11:10 a.m.)
Rotation 3 Presentations

Ting Liu
Energetic Study of Viral Genome Ejection
Advisor: Evilevitch

Madhumitha Ramesh
The Role of Ribosomal Protein L17 in Ribosome Assembly
Advisor: Woolford

Salini Konikkat
The Role of Ribosomal Protein L17 in Ribosome Assembly
Advisor: Woolford

Han Lai
Inferring Events in Species Trees with Short Branches
Advisor: Durand

Karen Kormuth
Regulation of the Patterning of the Sea Urchin Embryonic Skeleton
Advisor: Ettensohn

Zhongling Sun
Testing the Role of APC2 SAMP Repeats in Wnt Signaling in Drosophila Embryos
Advisor: McCartney

Olivia Molinar
Investigating the Role of Cell Cycle Dependent Phosphorylation in APC2 Activity
Advisor: McCartney

Eugenio Gallo
Understanding the Biology of Aging via Alternative Splicing Differences in Drosophila melanogaster
Advisor: Lopez