Spring 2010 Journal Club (Graduate Research Seminar)-Department of Biological Sciences - Carnegie Mellon University

Spring 2010 Journal Club

(Graduate Research Seminar)

Journal club is held in the Conference Room of the Mellon Institute at 12:30 p.m. on Wednesdays unless otherwise indicated.

Date

Speaker

Topic
1/13 Parker Mills
 Detection and Magnetic Measurement of Single Iron-Labeled Cells using MRI

Kaitlyn Dykstra Yip1A Structures the Mammalian Endoplasmic Reticulum
1/20 Jing Wen Pathway-specific Critical Periods for Experience-dependent Synaptic Strengthening in Developing Barrel Cortex
1/27 Smita Yadav
 Functional Role of Golgins in Membrane Motility

Qi Yan
 Development of Nanoscale Fluorescence Microscopy using Fluorogen Activating Proteins (FAPs)
2/3 Bradley Yates
 Design, Construction and Isolation of a Disulfide-free Single Chain Antibody for Use as a Cytoplasmic Detector of Protein-protein Interactions

Peter Berget, Ph.D.

Designing Protease Biosensors
Chen N, Zou J, Wang S, Ye Y, Huang Y , Gadda G and Yang JJ. Designing Protease Sensors for Real-Time Imaging of Trypsin Activation in Pancreatic Cancer Cells. Biochemistry 2009, 48, 3519–3526.

- and -

Xia Z, Xing Y, So MK, Koh AL, Sinclair R and Rao J. Multiplex Detection of Protease Activity with Quantum Dot Nanosensors Prepared by Intein-Mediated Specific Bioconjugation. Anal. Chem. 2008, 80, 8649–8655.
2/10 (Rescheduled for 3/3 due to weather.)
 Shantanu Bhattacharyya NMR study of Single Chain Antibody (ScFv) M8 Light Chain

John Holleran Cell Surface Detection of Proteins Implicated in Cystic Fibrosis Pathology
2/17
(12:30 - 2:30 p.m.)		 Rotation 2 Reports

Sailatha Ravi
Cloning, Expression and Characterization of Microtubule Depolymerizing Kinesin-13 MCAK Protein
Rotation Advisor: Hackney

Ashita Magal
PKA Controls Endosomal Actin Assembly to Regulate B2AR Recycling
Rotation Advisor: Puthenveedu

Androo Markham
Internal(ization) Affairs: Selective Endocytosis of β2 Adrenergic and δ-Opioid Receptors
Rotation Advisor: Puthenveedu

Phu Van
miRNAs in the Sea Urchin Developmental Gene Network
Rotation Advisor: Hinman

Idil Ulengin
PEGylation of Cysteines to Predict the Membrane Topology of YIP1A
Rotation Advisor: Lee

Stephanie Hughes
An Analysis of Histone Modifications and Splice Sites
Rotation Advisor: Schwartz

Michael Gamalinda
Key Players in Microtubule-mediated Extension of the Endoplasmic Reticulum
Rotation Advisor: Lee

Nathan Hsu
Uptake of PNAs into Schneider cells
Rotation Advisor: Lopez

Gregory Smith
High Dynamic Range Imaging: Shedding Light on the Dark Side of the Gel
Rotation Advisor: Minden

Rachel Vistein
Furthering Furrow Formation
Rotation Advisor: Minden
2/18
(12:30 - 2:30 p.m.)		 Rotation 2 Reports

Deborah Makin
The Role of the Myristoylated Tail in GRASP Proteins
Rotation Advisor: Linstedt

Amanda Soohoo
The Binding of a G-Quadruplex Forming 5'UTR RNA Sequence with Peptide Nucleic Acid
Rotation Advisor: Armitage

Andrew Kehr
HSQC: Heteronuclear Single Quantum Coherence or Human Suffering Quantified in a Cold Room?
Rotation Advisor: Rule

James Hughes
Who's Your ADAD?
Rotation Advisor: Macbeth

Paul Barton
Assaying the Activity of ADAR2
Rotation Advisor: Macbeth

Ritika Tewari
Role of Twist/ Snail in Wnt –dependent Apical Constriction
Rotation Advisor: McCartney

Suchitra Ramachandran
Does RLP7 Act as a Placeholder for RPL7?
Rotation Advisor: Woolford

Simran Saini
Characterization of Ribosomal Assembly Factor Ebp2
Rotation Advisor: Woolford

Mingrui Zhang
A Search for FAPs that Activate Long-wavelength Fluorogens
Rotation Advisor: Waggoner
2/24
 No Journal Club
3/3
 Shantanu Bhattacharyya NMR study of Single Chain Antibody (ScFv) M8 Light Chain

John Holleran Cell Surface Detection of Proteins Implicated in Cystic Fibrosis Pathology
3/10 No Journal Club
3/17 Jeanne Morin-Leisk Atlastins are Required for ER Structure

Dannie Durand, Ph.D.
 Why Does Yeast Need Three G1 Cyclins? What I did on my Sabbatical
3/24 Emily Furbee
 A Genetic Approach to Studying the Proteasome’s Role in Ventral Furrow Formation

Ashrifia Gogo
 Regulation of Skeletal Morphogenesis in the Sea
Urchin Embryo
3/31 Anmol Grover
 A Genetically Targetable Cell Surface Selected pH Sensor

Charles Ettensohn, Ph.D.
 Transcriptional Memory
Blobel GA, Kadauke S, Wang E, Lau AW, Zuber J, Chou MM, Vakoc CR. A reconfigured pattern of MLL occupancy within mitotic
chromatin promotes rapid transcriptional reactivation following mitotic exit. Mol Cell. 2009 Dec 25;36(6):970-83.
4/7 Timothy Jarvela
 Irradiation-induced Protein Inactivation Reveals Acute Requirements for Biogenesis of the Golgi Apparatus

Ayshwarya Subramanian Understanding Tumor Progression through Unmixing Approaches
4/14
 Kiran Rafiq
 An Analysis of the Skeletogenic Gene Regulatory Network of the Sea Urchin Embryo

Merissa Remus
 The Neurobiology of Cue-induced Reward-seeking Behavior
4/21 Megha Kapur

Dissecting the Role of APC2 in the Destruction Complex during Wnt Signaling

Jesse Sheehan Impact of Global vs. Local Attention on Neuronal Activity in Inferotemporal Cortex
4/28 Jason Talkish
 Determining the Chronology of Ribosome Assembly and Understanding the Mechanisms that Regulate It

David Whitney
 Exploring Neural Plasticity in the Visual Cortex
5/5
(12:30 - 2:30 p.m. in the Mellon Institute Social Room)
 Rotation 3 Presentations

Paul Barton
Analyzing the Release of Ribosomal Assembly Factors during Ribosome Biogenesis
Rotation Advisor: Woolford

James Hughes
Analyzing the Release of Ribosomal Assembly Factors during Ribosome Biogenesis
Rotation Advisor: Woolford

Phu Van
Investigating Inflammation with the Chick Chorioallantois Membrane Model
Rotation Advisor: Campbell

Rachel Vistein
Using Fluorescent Proteins to Visualize Cellular Events in Sea Urchins (or She Sees Sea Cells in Several Spectra)
Rotation Advisor: Ettensohn

Stephanie Hughes
Prediction of Transcription Factor Binding Sites in cis-Regulatory Modules
Rotation Advisor: Hinman

Sailatha Ravi
Optimization of mPEGylation to Predict the Topology of ER Membrane Proteins
Rotation Advisor: Lee

Ritika Tewari
Elucidating the Role of Atlastin-2 Coiled Coil Domain in the Endoplasmic Reticulum Network
Rotation Advisor: Lee

Idil Ulengin
GMAP210 has a Role in Membrane Motility
Rotation Advisor: Linstedt

Mingrui Zhang
Gaining a GRASP on Golgi Dynamics: Towards the Structure of a Golgi Reassembly Stacking Protein Mutant
Rotation Advisor: Linstedt

Ashita Magal
Role of Hsp27 in Cell Engulfment
Rotation Advisor: Minden
5/6
(12:30 - 2:30 p.m. in the Mellon Institute Social Room)
 Rotation 3 Presentations

Simran Saini
Characterization of a scFv-based Fluorogen Activating Protein
Rotation Advisor: Berget

Nathan Hsu
Crystallization of hADAR2
Rotation Advisor: Macbeth

Andrew Markham
Adenosine Deaminases That Act on tRNA
Rotation Advisor: Macbeth

Gregory Smith
Edge Statistics for Accurate Protein Family Clustering
Rotation Advisor: Durand

Andrew Kehr
Acute Inactivation of Actin Related Proteins: Sorting Out a New Model
Rotation Advisor: Puthenveedu

Amanda Soohoo
Agonizing Over Recycling
Rotation Advisor: Puthenveedu

Suchitra Ramachandran
Expectation in the Inferotemporal Cortex
Rotation Advisor: Olson

Michael Gamalinda
Sexy Alternatives Indeed
Rotation Advisor: Lopez

Deborah Makin
Recursive Splicing and RNA Polymerase Processivity
Rotation Advisor: Lopez