McNair Scholars 2005-Department of Biological Sciences - Carnegie Mellon University

McNair Scholars 2005

Tina JonasTina Jonas, The College of St. Scholastica
Mentor: Dr. Phil Campbell

Organizational Response of Mixed Cell Populations to Printed Patterns of FGF-2
Spatial and temporal patterns of growth factors direct cell migration, proliferation, and differentiation during wound healing. Gaining an understanding of how different cells respond to growth factor patterns will enable us to design more effective therapies for wound healing. The purpose of this project is to determine how different types of cells respond to FGF-2 patterns immobilized on fibrin. The goal of this project is to monitor the responses of multiple cell types to immobilized patterns of FGF-2 created using ink jet printing. In order to accomplish this I first needed to label the cell types so they could easily be distinguished from each other. This was done using different colored fluorescence by way of Quantum Dots to label the cytoplasm of each cell. The experiment was conducted using MG-63 human preosteoblastic cells and Swiss 3T3 mouse fibroblasts. The proliferative and migratory responses of the cells were monitored using time-lapse microscopy. Cells were successfully labeled with the Quantum Dots and placed simultaneously on a printed pattern. Time lapse imaging of the pattern showed a definite cellular response; however, an overly heavy cell population made the exact response difficult to distinguish. No manuscripts investigating how multiple cell types respond to immobilized growth factor patterns have previously been reported. These experiments will provide valuable knowledge for designing more effective wound healing therapies as well as basic knowledge for biology.