Equipment

Major equipment housed in the Molecular Biosensor and Imaging Center includes:
· MWG-AG Biotech Robot and controller with dedicated ST Robotics Arm and 96-well plate adapter
· Coulter EPICS Elite cell sorter with autoclone capacity
· UV/Vis spectrometer
· PTI fluorometer
· PTI Fluorescence Lifetime Fluorometer
· Semi-prep HPLC
· Chemical microwave
· Six Zeiss fluorescent microscope imaging systems
· Cellomics KineticScan high throughput imaging system
· Beckman Coulter high throughput imaging system
· Solamere Technology Group spinning disk confocal fluorescence imaging system consisting of a Yokogawa CSU10 Confocal Scanner Unit with a Laser Physics Reliant 100s-488 Ar laser and a Roper Scientific/Photometrics CoolSnapHQ cooled CCD camera mounted on an Olympus IX50 microscope

The Pittsburgh NMR Center for Biomedical Research features high-powered MRI magnets and MRS spectrometers:
· Bruker Avance 4.7-T/40-cm MRI scanner
· Bruker AVANCE DRX 7.0-Tesla/20-cm MRI scanner
· Bruker AVANCE 500WB 11.7-Tesla/8.9-cm MRI scanner
· A home-built Tecmag 2.35-T/31-cm MRI scanner
· Bruker MiniSpec 0.47 Tesla
· High Resolution NMR Spectrometers: 7.0-Tesla, 11.7-Tesla, 14.0-Tesla

The Electron Microscopy Facility
· Hitachi S-2460N Scanning Electron Microscope
· Quartz PCI Digital Image Acquisition Software
· Hitachi H-7100 Transmission Electron Microscope
· AMT Advantage 10 CCD Camera System and Digital Image Acquisition Software
· Reichert-Jung Ultracut E Ultramicrotome
· Reichert-Jung FC4E Low Temperature Sectioning System
· Pelco SC-6 Sputter Coater
· Pelko CPD2 Critical Point Dryer
· Leica- Reichert AFS Freeze Substitution System
· Baltec JFD 030 Jet Freeze Device
· Denton DV 502 Vacuum Evaporator

For more information about the EM facility, contact Joe Suhan jsgq@andrew.cmu.edu
412-268-6498