The research interest in the lab pertains to molecular mechanisms that establish and maintain the membrane bounded subcellular compartments of the secretory and endocytic pathways. This research area continues to be highly significant in terms of basic cell biology. It is also critical to understanding and treating human diseases such as cystic fibrosis, human immunodeficiency virus infection, and cancer.

Ongoing projects include: understanding the mode and mechanism of biogenesis of the Golgi apparatus, understanding the regulatory reactions that impinge on vesicle trafficking machinery to cause disassembly and reassembly of the Golgi apparatus during cell division, and understanding the interactions that occur within the Golgi and endocytic vesicle lumen to govern cell surface-to-Golgi trafficking of proteins and invasive toxins.

These projects are carried out in mammalian tissue culture cells using permeabilized cell assays, biochemical reconstitutions, cell imaging techniques and molecular genetic experiments.

Guo Y, Punj V, Sengupta D, and Linstedt AD. 2008. Coat-tether interaction in Golgi organization. Mol. Biol. of the Cell, 19, 2830–2843.

Feinstein NT and Linstedt AD. 2008. GRASP55 regulates Golgi ribbon formation. Mol. Biol. of the Cell, 19:2696–2707.

Gong H, Sengupta D, Linstedt AD and Schwartz R. 2008. Simulated de novo assembly of Golgi compartments by selective cargo capture during vesicle budding and targeted vesicle fusion. Biophys. J., 95: 1674–1688.

Feinstein T and Linstedt AD. 2007. Mitogen-activated Protein Kinase Kinase-dependent Golgi Unlinking Occurs in G2 phase and Promotes the G2/M Cell Cycle Transition. Mol. Biol. of the Cell, 18:594-604.

Bachert C, Fimmel CJ and Linstedt AD. 2007. Furin cleavage of cis-Golgi protein GP73 produces marker for liver disease. Traffic 8:1415-23.

Guo, Y and Linstedt, AD. COPII-Golgi Protein Interactions Regulate COPII Coat Assembly and Golgi size. J. Cell Biol. 174:53-63.

Puthenveedu MA, Bachert C, Puri S, Lanni F and Linstedt AD. GM130 and GRASP65-dependent Lateral Cisternal Fusion Confers Uniform Golgi Enzyme Distribution. Nature Cell Bio. 2006, 8: 238-248.

Puthenveedu MA, Linstedt AD. Subcompartmentalizing the Golgi Apparatus. Curr. Opin. Cell Bio. 2005; 17: 369-375.

Natarajan R and Linstedt AD. A cycling cis Golgi protein mediates endosome-to-Golgi traffic. Molecular Biology of the Cell 2004; 15: 4798-4806.

Linstedt AD. Positioning the Golgi Apparatus. Cell 2004; 118: 271-272.

Puthenveedu MA and Linstedt AD. Gene replacement reveals that p115/SNARE interactions are essential for Golgi biogenesis. Proceedings of the National Academy of Sciences USA 2004; 101: 1253-1256.

Puri S, Telfer H, Velliste M, Murphy R and Linstedt AD. Dispersal of Golgi matrix proteins during mitotic Golgi disassembly. Journal of Cell Science 2004; 117:451-456.

Puri S and Linstedt AD. Capacity of the Golgi apparatus for biogenesis from the endoplasmic reticulum. Molecular Biology of the Cell 2003; 14:5011-5018.