Jonathan W. Jarvik

Associate Professor

Education

Ph.D., Massachusetts Institute of Technology
Postdoctoral Appointment, Yale University

Research

CD-tagging

Efforts in my laboratory continue to advance CD-tagging, a method for genomic and proteomic discovery that generates cell lines that express proteins tagged with GFP or other reporters. Unlike other approaches for generating reporter-tagged cell lines, CD-tagging generates cells in which native transcriptional regulation and alternative splicing of the tagged genes are preserved.

The CD-tagging approach enables:

1. Discovery of previously unknown or poorly characterized proteins,
2. Observation and quantitation of the location and abundance of individual protein species in living cells and tissues,
3. Purification of tagged transcripts and proteins for biochemical and/or functional analysis,
4. Discovery and analysis of posttranslational modifications and protein-protein interactions, and
5. Development of new cell based assays.

Current work in the laboratory is focused on production and analysis of CD-tagged clones in mouse and human cultured cell lines and in mouse embryonic stem cells (mES cells).

FAP-based Biosensor Development

We have had considerable success in developing new live-cell assays for membrane protein translocation that employ FAP-based biosensors. FAPs are a new class of genetically encoded fluorescent tag developed in the Molecular Biosensor and Imaging Center (MBIC) here at CMU. FAP tags exhibit fluorescence only when bound to certain soluble fluorogens such as thiazole orange or malachite green.

Current work is focused on tracking protein translocation to or from the plasma membrane for a number of G-protein coupled receptors (GPCRs), and for the cystic fibrosis transmembrane conductance regulator (CFTR) and associated proteins. Additional efforts are aimed at developing new biosensors of protein proximity in living cells.

Publications

Holleran J, Brown D, Fuhrman MH, Adler SA, Fisher GW and Jarvik JW. Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells. Cytometry A, 77: 776-782, 2010.

Fisher GW, Adler SA, Fuhrman MH, Waggoner AS, Bruchez MP and Jarvik JW. Detection and quantification of B2AR Internalization in Living Cells using FAP-based biosensor technology. J. Biomolecular Screening, 15: 703-709, 2010.

Szent-Gyorgyi C, Schmidt BA, Creeger Y, Fisher GW, Zakel KL, Adler S, Fitzpatrick JA, Woolford CA, Yan Q, Vasilev KV, Berget PB, Bruchez MP, Jarvik JW and AS Waggoner. Fluorogen-activating single-chain antibodies for imaging cell surface proteins. Nat Biotechnol. 26:235-240, 2008.

Malehorn DE, Telmer CA, McEwen SB, An J, Kinsey AD, Retchless AC, Mason C, Vieta WM, Jarvik JW. Detection of cystic fibrosis mutations by peptide mass signature genotyping. Clinical Chemistry 49:1318-1330, 2003.

Jarvik, JW, Fisher GW, Shi C, Hennen L, Hauser C, Adler SA, Berget PB. In vivo functional proteomics: Mammalian genome annotation using CD-tagging. BioTechniques 34:852-866, 2002.