Peter B. Berget
Associate Professor Emeritus
246A Mellon Institute
Department of Biological Sciences
Carnegie Mellon University
4400 Fifth Avenue
Pittsburgh, PA 15213
Ph.D., University of Minnesota
Postdoctoral Appointment, Massachusetts Institute of Technology
My current research is focused in two main areas.
My laboratory is an active member of the new National Technology Center for Networks and Pathways. We are developing novel biosensors that will detect the activation of specific kinases, phosphatases, proteases and other signaling enzymes in mammalian cells. We are isolating and modifying single chain antibody molecules (scFvs) that bind and release fluorescent haptens as the platform for these biosensors.
My laboratory is also active in gene and protein discovery and genome annotation in mammalian cells. We are using "CD-tagging" technology as the main tool in this research effort. CD-tagging gives us the opportunity to identify and isolate new proteins or protein-containing structures and to identify the genes that encode them. High throughput methods are being developed to isolate tagged cells and identify the tagged gene. Tagged cell lines contain the fluorescent protein GFP and other "guest exons" that can be observed directly in the fluorescence microscope.
Zanotti KJ, Silva GL, Creeger Y, Robertson KL, Waggoner AS, Berget PB and Armitage BA. Blue fluorescent dye-protein complexes based on fluorogenic cyanine dyes and single chain antibody fragments. Org. Biomol. Chem., 2011, DOI: 10.1039/C0OB00444H.
Fitzpatrick JAJ, Yan Q, Sieber JJ, Dyba M, Schwarz U, Szent-Gyorgyi C, Woolford CA, Berget PB, Waggoner AS and Bruchez MP. STED nanoscopy in living cells using Fluorogen Activating Proteins. Bioconjugate Chem. 20 (10), 1843–1847, 2009.
Shank NI, Zanotti KJ, Lanni F, Berget PB, and Armitage BA. Enhanced Photostability of Genetically Encodable Fluoromodules Based on Fluorogenic Cyanine Dyes and a Promiscuous Protein Partner. J. Am. Chem. Soc. 131 (36), 12960–12969, 2009.
Falco CF, Dykstra KM, Yates BP, and Berget PB. scFv-based Fluorogen Activating Proteins and Variable Domain Inhibitors as Fluorescent Biosensor Platforms. Biotechnol. J. 4 (9), 1328-1336, 2009.
Özhalici-Ünal H, Pow CL, Marks SA, Jesper LD, Silva GL, Shank NI, Jones EW, Burnette JM 3rd, Berget PB and Armitage BA. A Rainbow of Fluoromodules: A Promiscuous scFv Protein Binds to and Activates a Diverse Set of Fluorogenic Cyanine Dyes. J Am Chem Soc. 130(38):12620-1, 2008 Sept 24.
Armitage BA and Berget PB. An Enlightening Structure-Function Relationship. Science, 319:1195-1196, 2008.
Szent-Gyorgyi C, Schmidt BA, Creeger Y, Fisher GW, Zakel KL, Adler S, Fitzpatrick JA, Woolford CA, Yan Q, Vasilev KV, Berget PB, Bruchez MP, Jarvik JW and Waggoner A. Fluorogen-activating single-chain antibodies for imaging cell surface proteins. Nature biotechnology, 26:235-240, 2008.
Jones EW, Berget PB, Burnette, III JM, Anderson C, Asafu-Adjei D, Avetisian S, Barrie F, Chen R, Chu B, Conroy SA, Conroy ST, Dill A, Eimer W, Garrity D, Greenwood A, Hamilton T, Hucko S, Jackson C, Livesey K, Monaco T, Onorato C, Otsuka M, Pai S, Schaeffer G, Shung S, Spath S, Stahlman J, Sweeney B, Wiltrout E, Yurovsky D and Zonneveld A. The Spectrum of Trp- Mutants Isolated as 5-Fluoroanthranilate Resistant Clones in Saccharomyces bayanus, Saccharomyces mikatae and Saccharomyces paradoxus. Yeast, 25(1):41-46, 2007.
Garcia Osuna E, Hua J, Bateman NW, Zhao T, Berget PB and Murphy RF. Large-Scale Automated Analysis of Location Patterns in Randomly Tagged 3T3 Cells. Ann Biomed Eng, 35(6):1081-1087, 2007 Jun.
Banerjee D, Markley AL, Yano T, Ghosh A, Berget PB, Minkley EG Jr, Khetan SK and Collins TJ. "Green" oxidation catalysis for rapid deactivation of bacterial spores. Angew Chem Int Ed Engl, 45(24):3974-7, 2006 Jun 12.
Highlander SK, Weissenberger S, Alvarez LE, Weinstock GM and Berget PB. Complete nucleotide sequence of a P2 family lysogenic bacteriophage, MhaA1-PHL101, from Mannheimia haemolytica serotype A1. Virology, 350(1):79-89, 2006.
Jarvik JW, Fisher GW, Shi C, Hennen L, Hauser C, Adler S and Berget PB. In vivo functional proteomics: Mammalian genome annotation using CD-tagging. BioTechniques, 33(4):852-866, 2002.